FM Minimal medium w/o Amino acids and w/o Ammonium chloride Size:1kg 16 pH (100 g/l) 4

16 pH (100 g/l) 4

MOPS 1 M 209

Peptone 5 Beef Extract 1 Yeast Extract 2 NaCl 5 Final pH 7

Methionine

FM Minimal medium w/o Amino acids and w/o Ammonium chloride Size:1kg 16 pH (100 g/l) 4Synthetic Defined Minimal Media FM Minimal medium w o Amino acids and w o Ammonium chloride This media is based on well defined formulations of mineral salts, vitamins and amino acids. Two commonly used synthetic defined minimal Dictystelium Discoideum media are FM and SIH. FM medium developed by Franke and Kessin is used for transformation of Dictyostelium Discoideum and genomic studies. This formulation supports the growth of most strains that are